Sperm DNA damage and seminal antioxidant activity in subfertile men

Supraphysiological ROS levels can lead to apoptosis, lipid peroxidation, and DNA protein damage. This pilot study aimed investigate the sperm oxidative damage in subfertile men, describe relationship between antioxidant system ROS. Sixty-four semen samples were categorised according evaluated routine parameters (WHO, WHO laboratory manual for examination processing of human semen, 2010). Results cross-referenced with [Comet (n = 53) TUNEL 49) assays], enzyme activity [SOD 51), CAT 48) GST 48)], content total thiols 36), hydroperoxides 35) MDA 31). Compared pathospermic samples, normozoospermic presented 40%–45% fewer spermatozoa fragmented DNA, 19% hydroperoxides, slightly higher levels. Asthenozoospermic/asthenoteratozoospermic had lowest activity. SOD showed a similar trend. Our results evidenced significant positive correlations immotile spermatozoa; CAT, thiols; GST; concentration; head/midpiece abnormalities hydroperoxides. work contributes existing body knowledge by showing that status correlates classic analysis parameters. Oxidative stress evaluation might be valuable diagnostic prognostic tool cases idiopathic male subfertility.